BSA–Boronic Acid Conjugate as Lectin Mimetics

Satya Nandana Narla; Poornima Pinnamaneni; Huan Nie; Yu Li; Xue-Long Sun

doi:10.1016/j.bbrc.2013.12.006

BSA–Boronic Acid Conjugate as Lectin Mimetics

Satya Nandana Narla
, Poornima Pinnamaneni
, Huan Nie
, Yu Li
, Xue-Long Sun

Cleveland State University
Harbin Institute of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

We report bovine serum albumin (BSA)–boronic acid (BA) conjugates as lectin mimetics and their glyco-capturing capacity. The BSA–BA conjugates were synthesized by amidation of carboxylic acid groups in BSA with aminophenyl boronic acid in the presence of EDC, and were characterized by Alizarin Red S (ARS) assay and SDS–PAGE gel. The BSA–BA conjugates were immobilized onto maleimide-functionalized silica beads and their sugar capturing capacity and specificity were confirmed by ARS displacement assay. Further, surface plasmon resonance (SPR) analysis of the glyco-capturing activity of the BSA–BA conjugates was conducted by immobilizing BSA–BA onto SPR gold chip. Overall, we demonstrated a BSA–BA-based lectin mimetics for glyco-capturing applications. These lectin mimetics are expected to provide an important tool for glycomics and biosensor research and applications.

Original language	American English
Journal	Biochemical and Biophysical Research Communications
Volume	443
DOIs	https://doi.org/10.1016/j.bbrc.2013.12.006
State	Published - Jan 10 2014

Disciplines

Biochemistry
Chemistry

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title = "BSA–Boronic Acid Conjugate as Lectin Mimetics",

abstract = " We report bovine serum albumin (BSA)–boronic acid (BA) conjugates as lectin mimetics and their glyco-capturing capacity. The BSA–BA conjugates were synthesized by amidation of carboxylic acid groups in BSA with aminophenyl boronic acid in the presence of EDC, and were characterized by Alizarin Red S (ARS) assay and SDS–PAGE gel. The BSA–BA conjugates were immobilized onto maleimide-functionalized silica beads and their sugar capturing capacity and specificity were confirmed by ARS displacement assay. Further, surface plasmon resonance (SPR) analysis of the glyco-capturing activity of the BSA–BA conjugates was conducted by immobilizing BSA–BA onto SPR gold chip. Overall, we demonstrated a BSA–BA-based lectin mimetics for glyco-capturing applications. These lectin mimetics are expected to provide an important tool for glycomics and biosensor research and applications.",

author = "Narla, \{Satya Nandana\} and Poornima Pinnamaneni and Huan Nie and Yu Li and Xue-Long Sun",

note = "Narla, S. N.; Pinnamaneni, P.; Nie, H.; Li, Y.; Sun, X. BSA–boronic acid conjugate as lectin mimetics. Biochem. Biophys. Res. Commun. 2014, 443, 562-567.",

year = "2014",

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day = "10",

doi = "10.1016/j.bbrc.2013.12.006",

language = "American English",

volume = "443",

journal = "Biochemical and Biophysical Research Communications",

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TY - JOUR

T1 - BSA–Boronic Acid Conjugate as Lectin Mimetics

AU - Narla, Satya Nandana

AU - Pinnamaneni, Poornima

AU - Nie, Huan

AU - Li, Yu

AU - Sun, Xue-Long

N1 - Narla, S. N.; Pinnamaneni, P.; Nie, H.; Li, Y.; Sun, X. BSA–boronic acid conjugate as lectin mimetics. Biochem. Biophys. Res. Commun. 2014, 443, 562-567.

PY - 2014/1/10

Y1 - 2014/1/10

N2 - We report bovine serum albumin (BSA)–boronic acid (BA) conjugates as lectin mimetics and their glyco-capturing capacity. The BSA–BA conjugates were synthesized by amidation of carboxylic acid groups in BSA with aminophenyl boronic acid in the presence of EDC, and were characterized by Alizarin Red S (ARS) assay and SDS–PAGE gel. The BSA–BA conjugates were immobilized onto maleimide-functionalized silica beads and their sugar capturing capacity and specificity were confirmed by ARS displacement assay. Further, surface plasmon resonance (SPR) analysis of the glyco-capturing activity of the BSA–BA conjugates was conducted by immobilizing BSA–BA onto SPR gold chip. Overall, we demonstrated a BSA–BA-based lectin mimetics for glyco-capturing applications. These lectin mimetics are expected to provide an important tool for glycomics and biosensor research and applications.

AB - We report bovine serum albumin (BSA)–boronic acid (BA) conjugates as lectin mimetics and their glyco-capturing capacity. The BSA–BA conjugates were synthesized by amidation of carboxylic acid groups in BSA with aminophenyl boronic acid in the presence of EDC, and were characterized by Alizarin Red S (ARS) assay and SDS–PAGE gel. The BSA–BA conjugates were immobilized onto maleimide-functionalized silica beads and their sugar capturing capacity and specificity were confirmed by ARS displacement assay. Further, surface plasmon resonance (SPR) analysis of the glyco-capturing activity of the BSA–BA conjugates was conducted by immobilizing BSA–BA onto SPR gold chip. Overall, we demonstrated a BSA–BA-based lectin mimetics for glyco-capturing applications. These lectin mimetics are expected to provide an important tool for glycomics and biosensor research and applications.

UR - https://engagedscholarship.csuohio.edu/scichem_facpub/506

U2 - 10.1016/j.bbrc.2013.12.006

DO - 10.1016/j.bbrc.2013.12.006

M3 - Article

VL - 443

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

ER -

BSA–Boronic Acid Conjugate as Lectin Mimetics

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