Chemoselective Immobilization of Biomolecules Through Aqueous Diels-Alder and PEG Chemistry

Xue-Long Sun; LiuChun Yang; Elliot L. Chaikof

doi:10.1016/j.tetlet.2008.02.111

Chemoselective Immobilization of Biomolecules Through Aqueous Diels-Alder and PEG Chemistry

Xue-Long Sun
, LiuChun Yang
, Elliot L. Chaikof

Emory University/Georgia Institute of Technology

Research output: Contribution to journal › Article › peer-review

Abstract

Aqueous Diels–Alder chemistry combined with a poly(ethylene glycol) (PEG) spacer was used to immobilize a diverse group of biomolecules onto a solid surface. Briefly, α, ω linear PEG conjugates were synthesized containing cyclopentadiene in the α position and either biotin, lactose, or protein A in the ω position. Linkers were coupled to N -maleimide (EMC)-functionalized glass substrates, and surface immobilization of biomolecules was confirmed by confocal fluorescence imaging.

Original language	American English
Journal	Tetrahedron Letters
Volume	49
DOIs	https://doi.org/10.1016/j.tetlet.2008.02.111
State	Published - Apr 14 2008

Keywords

Diels–Alder reaction; Chemical selective immobilization; Poly(ethylene glycol)
Biomolecule

Disciplines

Medicinal-Pharmaceutical Chemistry

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10.1016/j.tetlet.2008.02.111License: CC BY

http://dx.doi.org/10.1016/j.tetlet.2008.02.111

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title = "Chemoselective Immobilization of Biomolecules Through Aqueous Diels-Alder and PEG Chemistry",

abstract = " Aqueous Diels–Alder chemistry combined with a poly(ethylene glycol) (PEG) spacer was used to immobilize a diverse group of biomolecules onto a solid surface. Briefly, α, ω linear PEG conjugates were synthesized containing cyclopentadiene in the α position and either biotin, lactose, or protein A in the ω position. Linkers were coupled to N -maleimide (EMC)-functionalized glass substrates, and surface immobilization of biomolecules was confirmed by confocal fluorescence imaging.",

keywords = "Diels–Alder reaction; Chemical selective immobilization; Poly(ethylene glycol), Biomolecule",

author = "Xue-Long Sun and LiuChun Yang and Chaikof, \{Elliot L.\}",

note = "Sun, X.; Yang, L.; Chaikof, E. L. Chemoselective immobilization of biomolecules through aqueous Diels–Alder and PEG chemistry. Tetrahedron Lett. 2008, 49, 2510-2513.",

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doi = "10.1016/j.tetlet.2008.02.111",

language = "American English",

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T1 - Chemoselective Immobilization of Biomolecules Through Aqueous Diels-Alder and PEG Chemistry

AU - Sun, Xue-Long

AU - Yang, LiuChun

AU - Chaikof, Elliot L.

N1 - Sun, X.; Yang, L.; Chaikof, E. L. Chemoselective immobilization of biomolecules through aqueous Diels–Alder and PEG chemistry. Tetrahedron Lett. 2008, 49, 2510-2513.

PY - 2008/4/14

Y1 - 2008/4/14

N2 - Aqueous Diels–Alder chemistry combined with a poly(ethylene glycol) (PEG) spacer was used to immobilize a diverse group of biomolecules onto a solid surface. Briefly, α, ω linear PEG conjugates were synthesized containing cyclopentadiene in the α position and either biotin, lactose, or protein A in the ω position. Linkers were coupled to N -maleimide (EMC)-functionalized glass substrates, and surface immobilization of biomolecules was confirmed by confocal fluorescence imaging.

AB - Aqueous Diels–Alder chemistry combined with a poly(ethylene glycol) (PEG) spacer was used to immobilize a diverse group of biomolecules onto a solid surface. Briefly, α, ω linear PEG conjugates were synthesized containing cyclopentadiene in the α position and either biotin, lactose, or protein A in the ω position. Linkers were coupled to N -maleimide (EMC)-functionalized glass substrates, and surface immobilization of biomolecules was confirmed by confocal fluorescence imaging.

KW - Diels–Alder reaction; Chemical selective immobilization; Poly(ethylene glycol)

KW - Biomolecule

UR - https://engagedscholarship.csuohio.edu/scichem_facpub/56

UR - http://dx.doi.org/10.1016/j.tetlet.2008.02.111

U2 - 10.1016/j.tetlet.2008.02.111

DO - 10.1016/j.tetlet.2008.02.111

M3 - Article

VL - 49

JO - Tetrahedron Letters

JF - Tetrahedron Letters

ER -

Chemoselective Immobilization of Biomolecules Through Aqueous Diels-Alder and PEG Chemistry

Abstract

Keywords

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