Oriented Immobilized Sialyloligo-macroligand Microarray

Satya Nandana Narla; Xue-Long Sun

doi:10.1007/978-1-4939-3130-9_16

Oriented Immobilized Sialyloligo-macroligand Microarray

Satya Nandana Narla, Xue-Long Sun

Cleveland State University

Research output: Contribution to journal › Article › peer-review

Abstract

Silaic acid is the most common terminal glycan on cell surface glycoproteins and glycolipids, involving in many biological processes. Studying interactions between multivalent sialic acid scaffolds and proteins binding to it will give incredible information in understanding the biological process the sialic acid is involved in. Here we describe chemoenzymatic synthesis of chain-end functionalized sialyllactose-containing glycopolymers with different linkages and their oriented immobilization for glycoarray and SPR-based glyco-biosensor applications.

Original language	American English
Journal	Macro-Glycoligands: Methods and Protocols
DOIs	https://doi.org/10.1007/978-1-4939-3130-9_16
State	Published - Nov 8 2015

Keywords

Sialic acid
Glycan array
Glycopolymer
Lectin
Hemagglutinin
SPR

Disciplines

Biochemistry
Chemistry

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10.1007/978-1-4939-3130-9_16License: CC BY

http://link.springer.com/protocol/10.1007%2F978-1-4939-3130-9_16

Cite this

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title = "Oriented Immobilized Sialyloligo-macroligand Microarray",

abstract = " Silaic acid is the most common terminal glycan on cell surface glycoproteins and glycolipids, involving in many biological processes. Studying interactions between multivalent sialic acid scaffolds and proteins binding to it will give incredible information in understanding the biological process the sialic acid is involved in. Here we describe chemoenzymatic synthesis of chain-end functionalized sialyllactose-containing glycopolymers with different linkages and their oriented immobilization for glycoarray and SPR-based glyco-biosensor applications.",

keywords = "Sialic acid, Glycan array, Glycopolymer, Lectin, Hemagglutinin, SPR",

author = "Narla, \{Satya Nandana\} and Xue-Long Sun",

note = "Narla, S. N.; Sun, X. Oriented Immobilized Sialyloligo-macroligand Microarray. In Macro-Glycoligands: Methods and Protocols; Sun, X., Ed.; Springer New York: New York, NY, 2016; pp 195-206.",

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doi = "10.1007/978-1-4939-3130-9\_16",

language = "American English",

journal = "Macro-Glycoligands: Methods and Protocols",

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T1 - Oriented Immobilized Sialyloligo-macroligand Microarray

AU - Narla, Satya Nandana

AU - Sun, Xue-Long

N1 - Narla, S. N.; Sun, X. Oriented Immobilized Sialyloligo-macroligand Microarray. In Macro-Glycoligands: Methods and Protocols; Sun, X., Ed.; Springer New York: New York, NY, 2016; pp 195-206.

PY - 2015/11/8

Y1 - 2015/11/8

N2 - Silaic acid is the most common terminal glycan on cell surface glycoproteins and glycolipids, involving in many biological processes. Studying interactions between multivalent sialic acid scaffolds and proteins binding to it will give incredible information in understanding the biological process the sialic acid is involved in. Here we describe chemoenzymatic synthesis of chain-end functionalized sialyllactose-containing glycopolymers with different linkages and their oriented immobilization for glycoarray and SPR-based glyco-biosensor applications.

AB - Silaic acid is the most common terminal glycan on cell surface glycoproteins and glycolipids, involving in many biological processes. Studying interactions between multivalent sialic acid scaffolds and proteins binding to it will give incredible information in understanding the biological process the sialic acid is involved in. Here we describe chemoenzymatic synthesis of chain-end functionalized sialyllactose-containing glycopolymers with different linkages and their oriented immobilization for glycoarray and SPR-based glyco-biosensor applications.

KW - Sialic acid

KW - Glycan array

KW - Glycopolymer

KW - Lectin

KW - Hemagglutinin

KW - SPR

UR - https://engagedscholarship.csuohio.edu/scichem_facpub/293

UR - http://link.springer.com/protocol/10.1007%2F978-1-4939-3130-9_16

U2 - 10.1007/978-1-4939-3130-9_16

DO - 10.1007/978-1-4939-3130-9_16

M3 - Article

JO - Macro-Glycoligands: Methods and Protocols

JF - Macro-Glycoligands: Methods and Protocols

ER -

Oriented Immobilized Sialyloligo-macroligand Microarray

Abstract

Keywords

Disciplines

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