TY - JOUR
T1 - Oxygen Diffusion Through Natural Extracellular Matrices: Implications for Estimating "Critical Thickness" Values in Tendon Tissue Engineering
AU - Androjna, Caroline
AU - Gatica, Jorge E.
AU - Belovich, Joanne M.
AU - Derwin, Kathleen A.
N1 - Androjna, Caroline; Gatica, Jorge E.; Belovich, Joanne M.; Derwin, Kathleen A.. (2008). Oxygen Diffusion Through Natural Extracellular Matrices: Implications for Estimating "Critical Thickness" Values in Tendon Tissue Engineering. Tissue Engineering Part A 14, 559-569.
PY - 2008/4/1
Y1 - 2008/4/1
N2 - Oxygen is necessary for maintaining cell proliferation and viability and extracellular matrix (ECM) production in 3-dimensional tissue engineering. Typically, diffusion is the primary mode for oxygen transport in vitro; thus, ensuring an adequate oxygen supply is essential. In this study, we determined the oxygen diffusion coefficients of 3 natural ECMs that are being investigated as construct scaffolds for tendon tissue engineering: small-intestine submucosa (SIS), human dermis (Alloderm (R)), and canine fascia lata. Diffusion coefficients were determined using a standard diffusion cell system. The ranges for each matrix type were: SIS: 7 x 10(-6) - 2 x 10(-5) cm(2)/s, Alloderm (R): 1.9 - 3.1 x 10(-5) cm(2)/s, and canine fascia lata: 1.6 - 4 x 10(-5) cm(2)/s. We used the experimental oxygen diffusivity data for these natural ECMs in a mathematical model of oxygen diffusion through a cell-seeded scaffold to estimate the critical size of cell-seeded scaffold that can be cultured in vitro.
AB - Oxygen is necessary for maintaining cell proliferation and viability and extracellular matrix (ECM) production in 3-dimensional tissue engineering. Typically, diffusion is the primary mode for oxygen transport in vitro; thus, ensuring an adequate oxygen supply is essential. In this study, we determined the oxygen diffusion coefficients of 3 natural ECMs that are being investigated as construct scaffolds for tendon tissue engineering: small-intestine submucosa (SIS), human dermis (Alloderm (R)), and canine fascia lata. Diffusion coefficients were determined using a standard diffusion cell system. The ranges for each matrix type were: SIS: 7 x 10(-6) - 2 x 10(-5) cm(2)/s, Alloderm (R): 1.9 - 3.1 x 10(-5) cm(2)/s, and canine fascia lata: 1.6 - 4 x 10(-5) cm(2)/s. We used the experimental oxygen diffusivity data for these natural ECMs in a mathematical model of oxygen diffusion through a cell-seeded scaffold to estimate the critical size of cell-seeded scaffold that can be cultured in vitro.
UR - https://engagedscholarship.csuohio.edu/encbe_facpub/19
U2 - 10.1089/tea.2006.0361
DO - 10.1089/tea.2006.0361
M3 - Article
VL - 14
JO - Tissue Engineering Part A
JF - Tissue Engineering Part A
ER -