TY - JOUR
T1 - Quantification of Free Sialic Acid in Human Plasma through a Robust Quinoxalinone Derivatization and LC–MS/MS Using Isotope-labeled Standard Calibration
AU - Wang, Dan
AU - Zhou, Xiang
AU - Wang, Lin
AU - Wang, Sihe
AU - Sun, Xue-Long
N1 - Wang, D.; Zhou, X.; Wang, L.; Wang, S.; Sun, X. Quantification of free sialic acid in human plasma through a robust quinoxalinone derivatization and LC–MS/MS using isotope-labeled standard calibration. Journal of Chromatography B 2014, 944, 75-81.
PY - 2014/1/1
Y1 - 2014/1/1
N2 - We report an accurate quantification of free sialic acid (SA) in human plasma using LC–MS/MS method with isotope-labeled standard calibration (ILSC) and robust derivatization. Specifically, derivatization of SA with a stable and inexpensive 3,4-diaminotoluene (DAT) provides a stable product of SA with high MS response, proving a convenient and cost-effective LC–MS/MS analysis of free SA. In addition, the use of 13 C 3 -SA as calibration standard ensured the accuracy for the measurement. This assay used ultra high performance liquid chromatography (UHPLC) for separation of native/labeled SA and IS from matrix interference, and employed mass spectrometry in multiple reaction monitoring (MRM) mode for sensitive and selective detection. We have achieved a lower limit of quantification (LLOQ) of 20 ng/mL and a total running time of 4.2 min, which is the most sensitive and quick measurement for free SA in biomatrices.
AB - We report an accurate quantification of free sialic acid (SA) in human plasma using LC–MS/MS method with isotope-labeled standard calibration (ILSC) and robust derivatization. Specifically, derivatization of SA with a stable and inexpensive 3,4-diaminotoluene (DAT) provides a stable product of SA with high MS response, proving a convenient and cost-effective LC–MS/MS analysis of free SA. In addition, the use of 13 C 3 -SA as calibration standard ensured the accuracy for the measurement. This assay used ultra high performance liquid chromatography (UHPLC) for separation of native/labeled SA and IS from matrix interference, and employed mass spectrometry in multiple reaction monitoring (MRM) mode for sensitive and selective detection. We have achieved a lower limit of quantification (LLOQ) of 20 ng/mL and a total running time of 4.2 min, which is the most sensitive and quick measurement for free SA in biomatrices.
KW - Free sialic acid; Human plasma; Isotope-labeled standard calibration; Liquid chromatography–tandem mass spectrometry; Quinoxaline derivatization
UR - https://engagedscholarship.csuohio.edu/scichem_facpub/514
U2 - 10.1016/j.jchromb.2013.11.016
DO - 10.1016/j.jchromb.2013.11.016
M3 - Article
VL - 944
JO - Journal of Chromatography B
JF - Journal of Chromatography B
ER -