TY - JOUR
T1 - Serum Can Act as a Shear Protecting Agent in Agitated Hybridoma Cell Cultures
AU - Lee, Gyun Min
AU - Savinell (Belovich), Joanne M.
AU - Palsson, Bernhard O.
AU - Belovich, Joanne M.
PY - 1989/12/1
Y1 - 1989/12/1
N2 - Hybridoma cells, S3H5/γ2bA2, were grown in spinner flasks containing RPMI 1640 media at different levels of serum. Cells in low serum media (1% fetal bovine serum) were more sensitive to shear induced by mechanical agitation than cells in high serum media (10%), indicating that serum can protect cells from shear induced by mechanical agitation. The data suggest that serum alters the physiological properties of the cells to make them more shear-resistant, rather than by changing the physico-chemical properties of medium.
AB - Hybridoma cells, S3H5/γ2bA2, were grown in spinner flasks containing RPMI 1640 media at different levels of serum. Cells in low serum media (1% fetal bovine serum) were more sensitive to shear induced by mechanical agitation than cells in high serum media (10%), indicating that serum can protect cells from shear induced by mechanical agitation. The data suggest that serum alters the physiological properties of the cells to make them more shear-resistant, rather than by changing the physico-chemical properties of medium.
UR - https://engagedscholarship.csuohio.edu/encbe_facpub/157
UR - https://www.liebertpub.com/doi/abs/10.1089/hyb.1989.8.639?journalCode=hyb.1
U2 - 10.1089/hyb.1989.8.639
DO - 10.1089/hyb.1989.8.639
M3 - Article
VL - 8
JO - Hybridoma
JF - Hybridoma
ER -