Synthetic Melanin Films as Potential Interfaces for Peroxynitrite Detection and Quantification

Haitham F. Kalil; Shaimaa Mahera; Tiyash Bosed; Ousama Al-Mahmoude; Clara Kay; Mekki Bayachou

doi:10.1149/08010.1447ecst

Synthetic Melanin Films as Potential Interfaces for Peroxynitrite Detection and Quantification

Haitham F. Kalil
, Shaimaa Mahera
, Tiyash Bosed
, Ousama Al-Mahmoude
, Clara Kay
, Mekki Bayachou

Cleveland State University

Research output: Contribution to journal › Article › peer-review

Abstract

Peroxynitrite (PON) is a highly reactive oxygen-nitrogen species that facilitates both oxidation and nitration reactions. Early reports have revealed the deleterious effects of PON on DNA, proteins, and lipids. Recent studies have suggested that melanin can act as an antioxidative therapy to scavenge the reactive oxygen-nitrogen species (RO-NS) including PON. Melanin is a natural pigment that has many physiological functions involving the neutralization of highly oxidative species. In this project, the interaction between PON and synthetic melanin has been studied. In addition, the electrochemical characteristics of the polymerized 5,6-dihydroxy indole (DHI) as a model of synthetic melanin were examined using cyclic voltammetry and electrochemical quartz crystal microbalance (EQCM). The ultraviolet-visible (UV-Vis) spectroscopy showed a significant difference in the absorbance of PON alone and in the presence of melanin films. Finally, we report on the possibility of using the DHI-melanin film as a platform for the quantitative detection of PON in solutions.

Original language	American English
Journal	ECS Transactions
Volume	80
DOIs	https://doi.org/10.1149/08010.1447ecst
State	Published - Jan 1 2017

Disciplines

Chemistry

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Synthetic Melanin Films as Potential Interfaces for PeroxynitriteFinal published version, 1.21 MBLicense: CC BY

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@article{19078d1746ab4198b6867500f645bf33,

title = "Synthetic Melanin Films as Potential Interfaces for Peroxynitrite Detection and Quantification",

abstract = " Peroxynitrite (PON) is a highly reactive oxygen-nitrogen species that facilitates both oxidation and nitration reactions. Early reports have revealed the deleterious effects of PON on DNA, proteins, and lipids. Recent studies have suggested that melanin can act as an antioxidative therapy to scavenge the reactive oxygen-nitrogen species (RO-NS) including PON. Melanin is a natural pigment that has many physiological functions involving the neutralization of highly oxidative species. In this project, the interaction between PON and synthetic melanin has been studied. In addition, the electrochemical characteristics of the polymerized 5,6-dihydroxy indole (DHI) as a model of synthetic melanin were examined using cyclic voltammetry and electrochemical quartz crystal microbalance (EQCM). The ultraviolet-visible (UV-Vis) spectroscopy showed a significant difference in the absorbance of PON alone and in the presence of melanin films. Finally, we report on the possibility of using the DHI-melanin film as a platform for the quantitative detection of PON in solutions.",

author = "Kalil, \{Haitham F.\} and Shaimaa Mahera and Tiyash Bosed and Ousama Al-Mahmoude and Clara Kay and Mekki Bayachou",

note = "Kalil, H.; Maher, S.; Bose, T.; Al-Mahmoud, O.; Kay, C.; Bayachou, M., Synthetic Melanin Films as Potential Interfaces for Peroxynitrite Detection and Quantification. ECS Transactions 2017, 80 (10), 1447-1458.",

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month = jan,

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doi = "10.1149/08010.1447ecst",

language = "American English",

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T1 - Synthetic Melanin Films as Potential Interfaces for Peroxynitrite Detection and Quantification

AU - Kalil, Haitham F.

AU - Mahera, Shaimaa

AU - Bosed, Tiyash

AU - Al-Mahmoude, Ousama

AU - Kay, Clara

AU - Bayachou, Mekki

N1 - Kalil, H.; Maher, S.; Bose, T.; Al-Mahmoud, O.; Kay, C.; Bayachou, M., Synthetic Melanin Films as Potential Interfaces for Peroxynitrite Detection and Quantification. ECS Transactions 2017, 80 (10), 1447-1458.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Peroxynitrite (PON) is a highly reactive oxygen-nitrogen species that facilitates both oxidation and nitration reactions. Early reports have revealed the deleterious effects of PON on DNA, proteins, and lipids. Recent studies have suggested that melanin can act as an antioxidative therapy to scavenge the reactive oxygen-nitrogen species (RO-NS) including PON. Melanin is a natural pigment that has many physiological functions involving the neutralization of highly oxidative species. In this project, the interaction between PON and synthetic melanin has been studied. In addition, the electrochemical characteristics of the polymerized 5,6-dihydroxy indole (DHI) as a model of synthetic melanin were examined using cyclic voltammetry and electrochemical quartz crystal microbalance (EQCM). The ultraviolet-visible (UV-Vis) spectroscopy showed a significant difference in the absorbance of PON alone and in the presence of melanin films. Finally, we report on the possibility of using the DHI-melanin film as a platform for the quantitative detection of PON in solutions.

AB - Peroxynitrite (PON) is a highly reactive oxygen-nitrogen species that facilitates both oxidation and nitration reactions. Early reports have revealed the deleterious effects of PON on DNA, proteins, and lipids. Recent studies have suggested that melanin can act as an antioxidative therapy to scavenge the reactive oxygen-nitrogen species (RO-NS) including PON. Melanin is a natural pigment that has many physiological functions involving the neutralization of highly oxidative species. In this project, the interaction between PON and synthetic melanin has been studied. In addition, the electrochemical characteristics of the polymerized 5,6-dihydroxy indole (DHI) as a model of synthetic melanin were examined using cyclic voltammetry and electrochemical quartz crystal microbalance (EQCM). The ultraviolet-visible (UV-Vis) spectroscopy showed a significant difference in the absorbance of PON alone and in the presence of melanin films. Finally, we report on the possibility of using the DHI-melanin film as a platform for the quantitative detection of PON in solutions.

UR - https://engagedscholarship.csuohio.edu/scichem_facpub/484

U2 - 10.1149/08010.1447ecst

DO - 10.1149/08010.1447ecst

M3 - Article

VL - 80

JO - ECS Transactions

JF - ECS Transactions

ER -

Synthetic Melanin Films as Potential Interfaces for Peroxynitrite Detection and Quantification

Abstract

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